Biology

Areas of Interest:

• Morphology and condition indexes of diurnal raptors
• Gender ratios of red-tailed hawks in Nebraska
• Estimating raptor population density
• Use of indexes (road counts) as valid estimators of density
• Immune response to proteins modified by environmental exposures
• Garlic mustard as an invasive species

Degrees:

• B.S. (1991) - Texas Tech University, Wildlife Management
• M.S. (1993) - Texas Tech University, Microbiology
• PhD. (1996) - Texas Tech University, Biology

Professional Affiliations:

• The Wildlife Society
• Association of Field Ornithologists
• Raptor Research Foundation
• The Human Anatomy and Physiology Society

Dissertation & Thesis:

• Freeman, T. L.  1996.  Nucleotide sequence and regulation of the exuT complementing DNA of Erwinia chrysanthemi. Ph. D. Dissertation, Texas Tech University, Lubbock, Texas
• Freeman, T. L.  1993.  Cloning and characterization of the galacturonic acid uptake system in Erwinia chrysamhemi EC16. M. S. Thesis, Texas Tech University, Lubbock, Texas.

Publications:

• Schoenebeck, C. W., Turco, M., Fahrlander, R. M., Darveau, K. M., and Freeman, T. L.  2014.  Sex and age group specific changes in body condition of red-tailed hawks in central Nebraska.  The Wilson Journal of Ornithology  126(3):575-580.
• Freeman,  T. L.  2014.  Diurnal raptors of Buffalo County Nebraska.  The Prairie Naturalist  46:48-49.
• Freeman, T. L.  2011.  Monitoring the annual increase in garlic mustard in eastern Nebraska.  Proceedings of the 22nd North American Prairie Conference.  2011. 22:146-147.
• Freeman, T. L.  2010.  Riparian forest associated with tall-grass prairie: species composition and distribution along the Missouri River of central Nebraska.  North American Prairie Conference 21:63-68.
• Duryee, M. J., Freeman, T. L., Willis, M. S., Hunter, C. D., Hamilton, B. C., Suzuki, H., Tuma, D. J., Klassen, L. W., Thiele, G. M.  2005.  Scavenger receptors on sinusoidal liver endothelial cells (SECs) are involved in the uptake of aldehyde-modified proteins.  Molecular Pharmacology 68(5):1423-1430.
• Freeman, T. L., Haver, A., Duryee, M. J., Tuma, D. J., Klassen, L. W., Hamel. F. G., White, R. L., Rennard, S. I., & Thiele, G. M.  2005.  Aldehydes in cigarette smoke react with the lipid peroxidation product malonaldehyde to form fluorescent protein adducts on lysines.  Chemical Research in Toxicology 18(5):817-824.
• Freeman, T. L., Tuma, D. J., Thiele, G. M., Klassen, L. W., Worrall, S., Niemela, O., Parkkila, S., Emery, P. & Preedy, V. R.  2005.  Recent advances in alcohol-induced adduct formation. Alcoholism: Clinical and Experimental Research 29(7):1-7.
• Thiele, G. M., Hill, G. E., Pavlik, J. A., Freeman, T. L., Tuma, D. J., Duryee, M. J., & Klassen, L. W.  2005.  Halothane potentiates the alcohol-adduct induced TNF-alpha release in heart endothelial cells.  BMC Anesthesiology 5(1):3.
• Thiele, G. M., Duryee, M. J., Freeman, T. L., Sorrell, M. F., Willis, M. S., Tuma, D. J., & Klassen, L. W.  2005.  Rat sinusoidal liver endothelial cells (SECs) produce pro-fibrotic factors in response to adducts formed from the metabolites of ethanol.  Biochemical Pharmacology 70(11):1593-1600.
• Duryee, M. J., Klassen, L. W., Freeman, T. L., Willis, M. S., Tuma, D. J., & Thiele, G. M.  2004.  Lipopolysaccharide is a cofactor for malondialdehyde-acetaldehyde adduct-mediated cytokine/chemokine release by rat sinusoidal liver endothelial and kupffer cells.    Alcoholism: Clinical and Experimental Research 28(12):1931-1938.
• Duryee, M. J., Willis, M. S., Freeman, T. L., Kuszynski, C. A., Tuma, D. J., Klassen, L. W., & Thiele, G. M.  2004.  Mechanisms of alcohol liver damage: aldehydes, scavenger receptors, and autoimmunity.  Frontiers in Bioscience 9:3145-3155.
• Freeman, T. L., Thiele, G. M., Klassen, L. W., Klassen, B. T., & Mailliard, M. E.  2004.  N-(methylamino) isobutyric acid inhibits proliferation of CFSC-2C hepatic stellate cells.  Biochemical Pharmacology 68:223–230.
• Thiele, G. M., Freeman, T. L., & Klassen, L. W.  2004.  Immunologic mechanisms of alcoholic liver injury.  Seminars in Liver Disease 24(3):273-287.
• Thiele, G. M., Duryee, M. J., Willis, M. S., Sorrell, M. F., Freeman, T. L., Tuma, D. J., & Klassen, L.W.  2004.  Malondialdehyde-acetaldehyde (MAA) modified proteins induce pro-inflammatory and pro-fibrotic responses by liver endothelial cells.  Comparative Hepatology 14(3 Suppl 1):S25.
• Duryee, M. J., Freeman, T. L., Willis, M. S., Tuma, D. J., & Klassen, L. W.  2003.  Chronic ethanol consumption impairs receptor-mediated endocytosis of MAA-modified albumin by liver endothelial cells.  Biochemical Pharmacology 66:1045-1054.
• Freeman, T. L., Thiele, G. M., Tuma, D. J., Machu, T. K. & Mailliard, M. E.  2002.  ATA2-mediated amino acid uptake following partial hepatectomy is regulated by redistribution to the plasma membrane.  Archives of Biochemistry and Biophysics 400:215-222.
• Freeman, T. L., Kharbanda, K. K., Tuma, D. J., & Mailliard, M. E.  2002.  Inhibition of hepatic stellate cell collagen synthesis by N-(methylamino)isobutyric acid.    Biochemical Pharmacology 63(4):697–706.
• Zeitouni, N., Eubank, D. W., Lee, A. Q., Oxford, M. G., Freeman, T. L., Mailliard, M. E., & Beale, E. G.  2002.  Phosphoenolpyruvate carboxykinase is induced in growth-arrested hepatoma cells.   Biochemical and Biophysical Research Communications 290(5):1513-1520.
• Freeman, T. L., & Mailliard, M. E.  2000.  Post-transcriptional regulation of ATA2 transport during liver regeneration.  Biochemical and Biophysical Research Communications 288:729-732.
• Conrad, P. W., Freeman, T. L., Beitner-Johnson, D. & Millhorn, D. E.  1999.  EPASI trans-Activation during hypoxia requires p42/p44 MAPK .  Journal of Biological Chemistry 274(47):33709-33713.
• Freeman, T. L., Ngo, H. Q., & Mailliard, M. E.  1999.  Inhibition of system A amino acid transport and hepatocyte proliferation following partial hepatectomy in the rat.  Hepatology 30(2):437-444.
• Conforti, L., Kobayashi, S., Beitner-Johnson, D., Conrad, P. W., Freeman, T. L., & Millhorn, D. E.  1998.  Regulation of gene expression and secretory functions in oxygen-sensing pheochromocytoma cells.   Respiratory Physiology 115(2):249-260.
• Haseloff, B. J., Freeman, T. L., Valmeekam, V., Melkus, M. W., Oner, F., Valachovic, M. S., & San Francisco, M. J. D.  1998.  The exuT gene of Erwinia chrysanthemi EC 16: nucleotide sequence, expression, localization, and relevance of the gene product.   MPMI 11(4):270-276.
• Freeman, T. L., & San Francisco, M. J.  1994.  Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi EC16.  FEMS Microbiology Letters 118:101-106.

Courses this year:

• Introduction to Graduate Study (online for graduate students)
• Human Physiological Systems (online for graduate students)
• Essential Human Anatomy (online for graduate students)
• Functional Morphology (online for graduate students)
• Anatomy and Physiology II (Lab)